Main Article Content
Abstract
Pengembangan antioksidan alami dalam pemanfaatan pegagan (Centella asiatica) dan majapahit (Cresintia cujete) merupakan potensi yang besar. Penelitian ini telah melakukan optimasi komposisi ekstrak etanol pegagan (Centella asiatica) dan majapahit (Cresintia cujete) sebagai sumber antioksidan dengan dilakukan uji aktifitas penangkapan radikal bebas terhadap DPPH. Hasil penelitian menunjukkan bahwa baik ekstrak etanol pegagan (Centella asiatica) dan majapahit (Cresintia cujete) memiliki kandungan total fenol yang ditunjukkan dengan adanya kemampuan mereduksi reagen Folin Ciocalteu menjadi berwarna biru. Hasil optimasi komposisi ekstrak pegagan (Centella asiatica): majapahit (Cresintia cujete) = 30:70 memiliki aktifitas penangkapan radikal bebas terhadap DPPH yang paling baik dibandingkan ekstraks etanol pegagan (Centella asiatica) dan majapahit (Cresintia cujete). Nilai IC50 untuk komposisi ekstrak etanol pegagan (Centella asiatica): majapahit (Cresintia cujete) = 30:70 sebesar 0,103 mg/mL.
Keywords
Article Details
References
- Agarwal, M., and Chauhan, S., 2015, Anti-Mycobaterial Potential of Crescentia cujete (Bignoniaceae), International Journal of Advanced Research in Botany (IJARB), 1 (1), 1-9
- Ahda, M., 2014., Pengaruh Variasi Konsentrasi Etanol sebagai Pengekstrak Kulit Manggis terhadap Kandungan Fenol Total, Eksakta, 14, (2), 61-70
- Das, N., Islam, M.E., Jahan, N., Islam, M.S., Khan, A., Islam, M.R., and Parvin, M.S., 2014, Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds, BMC Complementary and Alternative Medicine, 14 (45), 1-9
- de Oliveira, A.M.F., Pinheiro, L.S., Pereira, C.K.S., Matias, W.N., Gomes, R.A., Chaves, O.S., de Souza, M.F.V., de Almeida, R.N., and de Assis, T.S., 2012, Total Phenolic Content and Antioxidant Activity of Some Malvaceae Family Species, Antioxidants, 1, 33-43
- Ejelonu BC., Lasisi AA., Olaremu AG,. and Ejelonu OC., 2011, The chemical constituents of calabash (Crescentia cujete), African Journal of Biotechnology, 10 (84), 19631-19636
- Herlina, 2010, Pengaruh Triterpen Total Pegagan (Centella asiatica(L)Urban) Terhadap Fungsi Kognitif Belajar dan Mengingat pada Mencit Jantan Albino(Mus musculus), Jurnal Penelitian Sains, 1006 (06), 20-24
- Kim, W-J., Kim, J., Veriansyah, B., Kim, J-D., Lee, Y-W., Oh, S-G., dan Tjandrawinata, R., 2009. Extraction of bioactive component from Centella asiatica using subcritical water. The Journal of Supercritical Fluids, 48:211-216.
- Mahbub, K.R., Hoq, M.M., Ahmed, M.M.,Sarker, A., 20011, In Vitro Antibacterial Activity of Crescentia Cujete and Moringa Oleifera, Bangladesh Research Publication Journal, 5 (4), 337-343
- Marxen, K., Vanselow , K.H., Lippemeier, S., Hintze, R., Ruser, A., and Hansen, U.P., 2007, Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements, Sensors, 7, 2080-2095
- Maurya, S., and Singh, D., 2010, Quantitative Analysis of Total Phenolic Content in Adhatoda vasica Nees Extracts, IJPRIF, 2, (4), 2403-2406
- Pietta P.G., 1999. Flavonoids antioxidants, Review, J. Nat. Prod., 63, 1035
- Sugiat, D., Hanani, E., Mun’im, A., 2010, Aktifitas Antioksidan dan Penetapan Kadar Fenol Total Ekstrak Metanol Dedak Beberapa Varietas Padi (Oryza Sativa L.), Majalah Ilmu Kefarmasian, VII (1), 24-33
- Sutrisno, E., Adnyana, I.K., Sukandar, E.Y., Fidrianny, I. dan Lestari, T., 2014, Kajian Aktivitas Penyembuhan Luka dan Antibakteri Binahong (Anredera cordifolia (Ten.) Steenis, Pegagan (Centella asiatica (L.) Urban) Serta Kombinasinya terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa dari pasien Luka Kaki Diabetes, Bionatura-Jurnal Ilmu-ilmu Hayati dan Fisik, 16, (2), 78 – 82
- Walker, J.M., 2002, The Protein Protocols Handbook, 2nd Edition, Humana Press Inc., Totowa, New Jersey
- Yung-chi, C., and Prusoff, W.H., 1973, Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction, Biochemical Pharmacology, 22, (23), 3099-3108
References
Agarwal, M., and Chauhan, S., 2015, Anti-Mycobaterial Potential of Crescentia cujete (Bignoniaceae), International Journal of Advanced Research in Botany (IJARB), 1 (1), 1-9
Ahda, M., 2014., Pengaruh Variasi Konsentrasi Etanol sebagai Pengekstrak Kulit Manggis terhadap Kandungan Fenol Total, Eksakta, 14, (2), 61-70
Das, N., Islam, M.E., Jahan, N., Islam, M.S., Khan, A., Islam, M.R., and Parvin, M.S., 2014, Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds, BMC Complementary and Alternative Medicine, 14 (45), 1-9
de Oliveira, A.M.F., Pinheiro, L.S., Pereira, C.K.S., Matias, W.N., Gomes, R.A., Chaves, O.S., de Souza, M.F.V., de Almeida, R.N., and de Assis, T.S., 2012, Total Phenolic Content and Antioxidant Activity of Some Malvaceae Family Species, Antioxidants, 1, 33-43
Ejelonu BC., Lasisi AA., Olaremu AG,. and Ejelonu OC., 2011, The chemical constituents of calabash (Crescentia cujete), African Journal of Biotechnology, 10 (84), 19631-19636
Herlina, 2010, Pengaruh Triterpen Total Pegagan (Centella asiatica(L)Urban) Terhadap Fungsi Kognitif Belajar dan Mengingat pada Mencit Jantan Albino(Mus musculus), Jurnal Penelitian Sains, 1006 (06), 20-24
Kim, W-J., Kim, J., Veriansyah, B., Kim, J-D., Lee, Y-W., Oh, S-G., dan Tjandrawinata, R., 2009. Extraction of bioactive component from Centella asiatica using subcritical water. The Journal of Supercritical Fluids, 48:211-216.
Mahbub, K.R., Hoq, M.M., Ahmed, M.M.,Sarker, A., 20011, In Vitro Antibacterial Activity of Crescentia Cujete and Moringa Oleifera, Bangladesh Research Publication Journal, 5 (4), 337-343
Marxen, K., Vanselow , K.H., Lippemeier, S., Hintze, R., Ruser, A., and Hansen, U.P., 2007, Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements, Sensors, 7, 2080-2095
Maurya, S., and Singh, D., 2010, Quantitative Analysis of Total Phenolic Content in Adhatoda vasica Nees Extracts, IJPRIF, 2, (4), 2403-2406
Pietta P.G., 1999. Flavonoids antioxidants, Review, J. Nat. Prod., 63, 1035
Sugiat, D., Hanani, E., Mun’im, A., 2010, Aktifitas Antioksidan dan Penetapan Kadar Fenol Total Ekstrak Metanol Dedak Beberapa Varietas Padi (Oryza Sativa L.), Majalah Ilmu Kefarmasian, VII (1), 24-33
Sutrisno, E., Adnyana, I.K., Sukandar, E.Y., Fidrianny, I. dan Lestari, T., 2014, Kajian Aktivitas Penyembuhan Luka dan Antibakteri Binahong (Anredera cordifolia (Ten.) Steenis, Pegagan (Centella asiatica (L.) Urban) Serta Kombinasinya terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa dari pasien Luka Kaki Diabetes, Bionatura-Jurnal Ilmu-ilmu Hayati dan Fisik, 16, (2), 78 – 82
Walker, J.M., 2002, The Protein Protocols Handbook, 2nd Edition, Humana Press Inc., Totowa, New Jersey
Yung-chi, C., and Prusoff, W.H., 1973, Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction, Biochemical Pharmacology, 22, (23), 3099-3108