The effect of kersen's skin infusion (Muntingia calabura L.) on blood uric acid levels of the rats (Rattus novergicus)

Asril Burhan, Usmar Usmar, Zulham Zulham, Ana Andarwiyati

Abstract

Background: Uric Acid or Gout is the results of purine metabolism, i.e. the enzymatic reshuffle of body cells from dinucleotide or ribonucleotide acids. High uric acid levels can be settled in the central joints and mesenchymal tissues such as the kidney.
Objective: To determine an effect of administering the Kersen's (Muntingia calabura L.) ripe skin infusion against uric acid levels in the rats which have been induced with caffeine.
Methods: Eighteen rats was divided into six groups, i.e. three as a control group (negative, positive and normal) and three as a treatment group (0,5%, 1%, and 2% doses). The measurements of blood uric acid levels were performed before induction, after induction and after treatment on 9th, 12nd, and 15th days.
Results: There was a reduction of the uric acid levels in the rats which administered by ripe Kersen's fruit skin infusion at 1% with 3,43 mg/dL and 2% with 3.32 mg/dL doses. However, this reduction is not equivalent to the effect of allopurinol control decreased at 2.9 mg/dL. Statistical analysis results with Complete Randomized Design (RAL) revealed that positive control group, infusion in 1% and 2% dose did not have a significant difference with normal controls, that means allopurinol and both doses test give an effect to return the uric acid to normal conditions.
Conclusion: The ripe Kersen's (Muntingia Calabura) fruit skin infusion provides a reduction effect on blood uric acid levels in the rats (Rattus Novergicus) that have been induced by caffeine. Concentration of infusion that reduced effect of blood uric acid levels in the rats are at 1% ( 1 g/ 200 gBW) and 2% (2 g/200 gBW). However, the reduction is not equivalent to the positive control of Allopurinol.

Keywords

Ripe Kersen's; Muntingia calabura L.; uric acid; Rats; Rattus novergicus

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References

Alexander D, Alam G, Kondar W. Pengaruh ekstrak rimpang temu Putih (Curcuma zedoaria) terhadap kadar asam urat pada kelinci. Majalah Farmasi dan Farmakologi. 2011;15(2):89–94.

Ellen MM, Caroline FT, John TC, Russ B, Teri EK. PharmGKB summary: Uric acid-lowering drugs pathway, pharmacodynamics. Pharmacogenetics and Genomics. 2014;24(9):464–76.

Muhtadi, Wahyuningtyas N, Syahprawira Y, Suhendi A. Penghambatan xantin Oosidase oleh kombinasi ekstrak Jinten Hitam (Nigella sativa L.)., Meniran (Phyllanthus niruri L.), dan Tempuyung (Sonchus ArvensisL.) pada mencit hiperurisemia. Symposium Nasional Kimia Bahan Alam. 2015;10–1.

Ida Kholifaturrokhmah, Ratna Damma Purnawati. Pengaruh pemberian esktrak buah (Muntingia calabura L.) dosis bertingkat terhadap gambaran histopatologi ginjal mencit BALB/C yang hiperurisemia. Jurnal Kedokteran Diponegoro. 2015;5(3).

Dewi Astuti. Efek antihiperurisemia ekstrak air kombinasi kelopak Rosella (Hibiscus sabdariffa L) dan akar tanaman akar kucing (Acalypha indica L) pada tikus putih jantan yang diinduksi kalium oksonat. Universitas Indonesia; 2011.

Meiliza ER. Pengaruh jus buah Kersen (Muntingia calabura L.) terhadap kadar asam urat darah mencit (MusMusculus). Universitas Muhammadiyah Surakarta; 2013.

Micaela G, Natalia M, Saverio M, Vincenzo M. The treatment of hyperuricemia. International Journal of Cardiology. 2016;213:23–7.