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Abstract

Mikroalga memiliki kandungan lemak (lipid) dan asam lemak (fatty acid) yang dapat dikonversi menjadi salah satu energi alternative biodiesel. Biodiesel adalah bahan bakar alternatif yang tidak beracun dan dapat terurai secara alami. Kelimpahan dan pertumbuhan mikroalga Chorella Sp. yang sangat cepat dinilai ideal dan potensial untuk dijadikan sebagai bahan baku produksi bioenergi. Disisi lain mikroalga membutuhkan nutrisi seperti karbon dan nitrogen untuk pertumbuhannya. Sumber nutrisi dapat diperoleh dari limbah cair, seperti limbah cair hotel. Tujuan dari penelitian ini adalah untuk menentukan kondisi terbaik untuk Chlorella sp memproduksi kandungan lipid dengan memvariasikan rasio C: N 100: 7, 100: 13, 100: 32 dan panjang gelombang cahaya menggunakan lampu cahaya putih (380-750 nm) , lampu biru (450-495 nm), lampu hijau (495-570 nm), dan lampu merah (620-750 nm) juga mengamati kemampuan Chlorella sp untuk mengurangi nutrisi dalam media limbah cair. Percobaan dilakukan pada suhu kamar di bawah pencahayaan 2000 lux selama 15 hari. Hasil penelitian menunjukkan bahwa Chlorella sp menghasilkan konten lipid tertinggi (36,84%) pada rasio C: N 100: 7. Selain itu, Ketika alga dikultivasi dengan panjang gelombang cahaya berbeda menunjukkan bahwa Chlorella sp tumbuh lebih baik dan menghasilkan kandungan lipid sebesar 40,91% di bawah cahaya biru (450-495 nm) bila dibandingkan dengan jenis cahaya lainnya.

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